The spatial measurements include location, area, perimeter and moments for calculating a fitting ellipse. (5) Particle analysis is the operation where the filtered binary image is analyzed by quantifying various spatial properties of different "particles" (i.e., spots or regions) in the image. Typical morphological filters include: erosion/dilation, opening/closing, tophat and watershed. (4) Morphological filtering usually follows the threshold operations but some morphological operations can actually precede the threshold step. Download the fully-functional Igor Pro 9 and try it free for 30 days. Igor has been used by tens of thousands of technical professionals since its introduction in 1989. (3) Threshold operation to convert the image from a gray-scale to a binary form. Igor Pro is an interactive software environment for experimentation with scientific and engineering data and for the production of publication-quality graphs and page layouts. (2) Image filtering (cleaning up the image to improve S/N ratio) can be accomplished using localized filters or mathematical transforms. (1) image transformations and color conversions where the acquired image is converted into standard form in colorspace and in range. The conventional approach to image processing involves the following steps: Being able to display images in false color or using a non-linear level mapping is sometimes helpful when trying to visually analyze images. Image display can be as simple as placing an RGB image in a graph window or as complicated as creating an overlay of multiple images combined with contour lines and legend. Rounding up the list of built-in operations is MatrixOP which provides efficient means for formulating and performing mathematical operations on images. In addition to the dedicated ImageXXX operations you can also take advantage of general analysis functions such as FFT and curve fitting in image processing applications. The latter are combined as the Image Processing Package which you can load from Analysis menu. The main component of the image processing tools are the ImageXXX operations which are supplemented by the image processing procedure files. The processing and analysis stage depends on the nature of the images and the information of interest. In both cases the images can be displayed on the screen for visual inspection and analysis or they could be automatically analyzed without user intervention. Image acquisition can be as simple as loading multi-dimensional data from disk file or as complicated as using an XOP to grab live video frames to disk (see XOP Toolkit for information on creating your own XOP). Igor Pro® contains a full set of operations and functions for scientific image analysis applications which make it an ideal cross-platform tool for image acquisition, display and processing.
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